Figure 4.

M1 macrophage genes are more abundantly expressed in HS skin. (A) Dot plot showing the percent expressed and average expression of select M1 macrophage genes between key cellular populations in HS skin. (B) Dot plot showing the percent expressed and average expression of select M1 macrophage genes between HS and HC skin in monocyte/macrophage populations. (C) Dot plot showing the percent expressed and average expression of select M2 macrophage genes between HS and HC skin in monocyte/macrophage populations. (D) qPCR of GBP1 (log₂FC = 3.87, **p < 0.01), GBP5 (log₂FC = 6.33, ***p < 0.005), IRF1 (log₂FC = 2.58, **p < 0.01), and STAT1 (log₂FC = 3.38, **p < 0.01) in HS lesions and HC skin. log₂FC is expressed as an average of skin samples from 3 HS or HC patients relative to HC skin. Measurements were collected in duplicate. Data is represented as the mean log₂FC value +/– standard error of the mean. cDC, conventional dendritic cell; HC, healthy control; pDC, plasmacytoid dendritic cell.