Figure 3.

(A–C) Decreased frequencies and numbers of T-bet⁺CD11c^hi B cells in Cd38^−/− cGVHD mice. (D–F) Decreased anti-ssDNA autoantibody serum levels in Cd38^−/− cGVHD mice. Correlation with the frequencies of T-bet⁺CD11c^hi B cells. (A) Representative flow cytometry plot showing T-bet⁺CD11c^hi cells and T-bet^−/loCD11c⁻ on gated CD19⁺ B cells from spleen. (B) Percentages of CD11c^hiT-bet⁺ cells (of CD19⁺ B cells). Mean ± SEM. P-values are shown for Welch’s t-test. (C) Total number of CD11c^hiT-bet⁺ cells. Mean ± SEM. P-values are shown for Welch’s t-test. (D) Anti-ssDNA serum levels. Median values. P-values are shown for Mann–Whitney test. (E) Total IgG serum levels. Median values. P-values are shown for Mann–Whitney test. (F) Positive correlation between anti-ssDNA serum levels from cGVHD mice and the frequencies of CD11c^hiT-bet⁺ B cells (simple linear regression). The anti-ssDNA levels and frequencies are shown in log scale. The results are cumulative data from two to four different experiments per time point and mouse type, each with three to five mice per experiment. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, *****P < 0.00001.