Figure 9.

Representative microphotographs of the histological studies performed on the spleen, liver, and kidney 3-μm sections at 2 weeks of the adoptive transfer of spleen cells from female bm12 to female WT (left panels) or to Cd38^−/− (right panels) mice. (A, B) Spleen, (C, D) liver, and (E–J) kidney. (A–D) Spleen and liver tissue sections stained with H&E. (E–H) Kidney tissue sections stained with PAS. (I, J) Kidney sections stained with Masson’s trichrome. Images were taken with a BH2 Olympus microscope. (A, B) ×20 magnification; (C–F) ×10 magnification; (G–J) 40× magnification. (H, J) Scale bar: 50 μm. Note in WT cGVHD mice and Cd38^−/− cGVHD mice the presence of mild perivascular chronic inflammatory infiltrate in liver [black head arrow in (C) and (D)]. Note in WT cGVHD mice hyaline casts in kidney [black arrow in (E)], periglomerular chronic inflammatory infiltrate [black and white arrow in (I)], and increased glomerular size [asterisks in (G) and (I)].