TABLE 1.
Algal health parameters of Chaetoceros calcitrans (CC) and Tisochrysis lutea (T-Iso) cultures which had been ‘stressed’ under high pH conditions or, were non-stressed (Control) before feeding to oyster larvae.
| CC |
T-Iso |
|||
| Control | Stressed | Control | Stressed | |
| pH | 7.5 | 8.98*** | 7.6 | 8.75*** |
| (± 0.1) | (± 0.16) | (± 0.14) | (± 0.12) | |
| Cell density (106 cells mL–1) | 24 | 5.7* | 5.8 | 2.4** |
| (± 3.6) | (± 0.64) | (± 0.6) | (± 1.2) | |
| Quantum yield (Fv’/Fm’) | 0.72 | 0.52*** | 0.72 | 0.62*** |
| (± 0.01) | (± 0.03) | (± 0.01) | (± 0.01) | |
| Fluorescence (Ft) | 5729 | 2093** | 3870 | 1453* |
| (± 638) | (± 343) | (± 618) | (± 98) | |
| Size (a.u.) | 45.51 | 80.55* | 99.36 | 115.68 |
| (± 2.37) | (± 8.32) | (± 7.63) | (± 7.55) | |
| Complexity (a.u.) | 9.48 | 35.19** | 16.83 | 18.22# |
| (± 0.30) | (± 4.65) | (± 0.25) | (± 0.52) | |
| Chlorophyll FL3 (a.u.) | 14.18 | 19.13*** | 23.55 | 21.53 |
| (± 0.48) | (± 0.38) | (± 1.05) | (± 1.14) | |
| ROS production (a.u.) | 4.73 | 30.75# | 12.82 | 12.38 |
| (± 1.34) | (± 18.59) | (± 0.72) | (± 0.32) | |
| Viability (%) | 99.86 | 89.42* | 98.96 | 97.86 |
| (± 0.06) | (± 3.97) | (± 0.35) | (± 0.7) | |
| Neutral Lipid (a.u.) | 4.08 | 22.34* | 17.89 | 16.75 |
| (± 0.12) | (± 3.60) | (± 1.15) | (± 0.84) | |
Results are expressed as the mean (± SEM) of 4 carboys using time as replication (i.e., day 0, 1, 2, and 3). Asterisks indicate significant effect of treatment at: #p < 0.1; *p < 0.05; **p < 0.01; ***p < 0.001 using t-tests. ROS, reactive oxygen species; a.u., arbitrary units.