FIGURE 1.

Characterization of Shank3 immunoreactivity (IR) in the olfactory bulb (OB) and frontal lobe cortex. Panels (A,B) are images of medial frontal cortex from adjacent sections processed identically except for the omission of the primary antibody in the latter, which resulted in the lack of IR across the entire section (B). (C) shows light diffuse IR across the OB, with the framed area enlarged as (D), illustrating the labeling in individual glomeruli. (E–H) are low and high-power views of the labeling in the lateral frontal cortex, and (I–L) show IR in the anterior cingulate neocortex (ACC). Shank3 IR exhibits largely a neuropil-type pattern with the intensity noticeable higher in layers II–IV and VI than I and V. An apparently subset of pyramidal and multipolar neurons shows greater IR than the neuropil, which can be better recognized in the immunolabeled section with hematoxylin counterstain (L). Additional abbreviations: CC, corpus collosum; NFL, nerve fiber layer; GL, glomerular layer; EPL, external plexiform layer; GCL, granule cell layer; WM, white matter; I–VI, cortical layers. Scale bars and panel orientation are as indicated in the panels.