Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 24;13:717263. doi: 10.3389/fnagi.2021.717263

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Wan, Ai, Yang, Jiang, Zhang, Luo, Huang, Tu, Pan, Tu, Manavis, Xiao and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Western blot characterization of the Shank3 antibody and assessment of the Shank3 protein levels in representative brain regions. (A) Schematically illustrates the adenovirus genomic structure, including manipulating apparatus and the human SHANK3 cDNA. (B) shows an example of in vivo infection in the rat hippocampus 30 days post unilateral stereotactical injection of the constructed virus. (C) shows a representative immunoblot image, illustrating the relative levels of Shank3 protein in the hippocampal lysates from rats infected with the constructed virus (Shank+) and vehicle virus, and postmortem human brain. The major immunoblotted products migrate at ∼180 kDa in all samples; several light bands also migrated from 90 to 180 kDa, with an apparently greater amount in the Shank + transinfected sample. (D) shows immunoblotted products of Shank3, PSD95, and β-actin (internal loading control) in lysates from different brain regions from three cases, as indicated. (E) Plots the relative levels of Shank3 and PSD95, expressed as optic density (o.d.) ratios normalized to internal control across regions. (F) shows a positive correlation between Shank3 and PSD95 levels based on the multi-region and multi-case immunoblotting data. Abbreviations of brain regions in (D) are as indicated correspondingly in (E).