TABLE 1.

Demographic information of brain donors and staging of Alzheimer-type neuropathoplogy.

Case	Group	Age (y)	Sex	Clinical diagnosis or cause of death	Postmortem delay (h)	Braak NFT stage	Thal Aβ phase	Tissue usage
1	Youth	12	F	Thalassemia	12	N/A*	N/A	IHC**
2	Youth	14	M	Leukemia	9	N/A	N/A	IHC/WB**
3	Youth	14	F	Tuberculosis	10	N/A	N/A	IHC
4	Youth	16	F	Transposition of the great arteries	8	N/A	N/A	IHC
5	Youth	19	M	Infection/sepsis	11	N/A	N/A	IHC/WB**
6	Youth	19	M	Cardiopulmonary failure	5	N/A	N/A	IHC/WB**
7	Youth	22	F	Lupus erythematosus	12	N/A	N/A	IHC/WB**
8	Adult	31	F	Vagina cancer	10	0	0	IHC
9	Adult	38	M	Liver cancer	8	0	0	IHC/WB**
10	Adult	49	M	Lung cancer	10	0	0	IHC/WB**
11	Adult	50	M	Lung cancer	7	0	0	IHC/IF/WB**
12	Adult	50	F	Myocardial infarction	12	0	0	IHC/WB**
13	Adult	55	M	Liver cancer	8	0	0	IHC**/IF
14	Aged	68	M	Renal failure	8	II	0	IHC/WB**
15	Aged	70	F	Pneumonia	12	II	0	IHC
16	Aged/AD***	74	M	Multisystem failure, demented	5	V	5	IHC**
17	Aged	76	M	Heart stroke	7	0	0	IHC/IF
18	Aged	77	M	Multisystem failure	12	III	0	IHC/WB**
19	Aged	80	M	Iron-deficiency anemia	7	III	0	IHC/WB**
20	Aged	80	M	Lung cancer	15	III	1	IHC
21	Aged	81	F	Chronic renal failure	7	IV	0	IHC**
22	Aged	81	M	Cardiovascular failure	6	II	0	IHC/WB**
23	Aged/AD	83	F	Multisystem failure, demented	12	III	4	IHC/WB**
24	Aged/AD	85	M	Astrocytoma, demented	12	III	4	IHC**
25	Aged	86	M	COPD****	6	III	0	IHC/WB**
26	Aged/AD	87	F	Coronary heart disease, demented	6	VI	5	IHC/WB**
27	Aged/AD	88	F	Heart failure, demented	9	V	4	IHC/WB**
28	Aged	95	M	Multisystem failure	14	III	0	IHC

*AD-type neuropathology not assessed; **Used for assessing regional and age-related change in Shank3 expression; ***Cases met the criteria for neuropathological diagnosis of AD (Braak and Braak, 1991; Thal et al., 2002; Braak et al., 2006; Montine et al., 2012); ****chronic obstructive pulmonary disease.

IHC, immunohistochemistry; IF, immunofluorescence; WB, western blot.