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Validation of candidate biomarkers for BC prognosis using WB. WB of protein in urine samples was used to compare candidate BC biomarkers in (A) normal control vs. preoperative BC, (B) preoperative BC vs. common urinary diseases, and (C) preoperative BC vs. postoperative BC samples. (D) Quantification of protein abundance. The total protein content of each sample was used as the loading control. *, P<0.05, and ***, P<0.001. BC, bladder cancer; WB, western blot.
