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Expression analysis of PI3K/Akt/HIF-1α pathway. (A) VEGFA, Akt, p-Akt and HIF-1α were determined by western-blot after morphine treatment. GAPDH was used as loading control. (B, C) VEGFA, Akt, p-Akt and HIF-1α were determined by western blot after the inhibitor LY294002 inducement. GAPDH was used as loading control. (D) VEGFA in the CM treated with LY294002 was assessed by ELISA. **, P<0.01; ***, P<0.001.
