Skip to main content
. 2021 Aug;12(4):1625–1642. doi: 10.21037/jgo-21-467

Figure 4.

Figure 4

Combined application of CTX and PP242 inhibited the phosphorylation of key molecules of EGFR downstream signaling pathways in Caco-2 cells. Caco-2 cells were untreated or treated with 20 µg/mL CTX and 1 µmol/L PP242 alone or in combination for 96 h. (A) The protein content of cellular total and phosphorylated EGFR, MEK, ERK, MKK and JNK contents were determined by western blotting. (B) The semi-quantitative analysis of cellular total and phosphorylated EGFR, MEK, ERK, MKK and JNK contents was performed based on the relative density values of the bands of western blotting. The relative density value of the control group was set to one. (C) qPCR was used to measure the mRNA expression of EGFR, MEK, ERK, MKK and JNK in different treatment groups relative to the control group. The relative mRNA expression of the control group was set to one. Each bar represents the mean ± SEM. All data were derived from at least three separate experiments. *, P<0.05; **, P<0.01. CTX, cetuximab; EGFR, epidermal growth factor receptor; MEK, mitogen-activated protein kinase kinase; ERK, extracellular regulated protein kinase; MKK, MEK 4/7; JNK, c-Jun N-terminal kinase; qPCR, quantitative real time polymerase chain reaction; SEM, standard error of mean.