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. 2021 Aug;9(15):1245. doi: 10.21037/atm-21-3153

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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The inhibitory effects of caffeine on the viability, proliferation, migration, and pluripotency of BMSCs. (A) Chemical structures of caffeine. (B) The CCK-8 assay was used to assess the cell viability of BMSCs after treatment with caffeine at 0.01, 0.05, 0.1, 0.5, and 1.0 mM for 48 h. (C) The colony formation assay was performed to determine the proliferative ability of BMSCs treated with 0.01, 0.05, 0.1, 0.5, and 1.0 mM caffeine (100×). (D) The statistical results of the colony formation assay. (E) The migration of BMSCs in the presence of caffeine was determined by the wound healing assay (100×). (F) Quantitative analysis of the wound healing assay. (G) QRT-PCR analysis was applied to measure the mRNA expression levels of pluripotency-associated transcription factors, including Nanog, Sox2 and Oct4. n=3. *, P<0.05; **, P<0.01; ***, P<0.001. BMSCs, bone marrow mesenchymal stem cells.