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. 2021 Aug;9(15):1245. doi: 10.21037/atm-21-3153

Figure 6.

Figure 6

PL reversed the effects of caffeine in BMSCs via the ER pathway. (A) Chemical structures of ICI. (B) ARS staining was performed to detect the osteogenesis of BMSCs in the presence of caffeine, caffeine + PL, and caffeine + PL + ICI (100×). (C) Quantitative analysis of ARS staining. (D) ALP staining was used to measure the osteogenesis of BMSCs in the presence of caffeine, caffeine + PL, and caffeine + PL + ICI (100×); (E) Quantitative analysis of ALP staining. (F) QRT-PCR analysis was applied to assess the expression of Runx2, Osterix, and OCN in BMSCs after treatment with caffeine, caffeine + PL, and caffeine + PL + ICI. (G) ORO staining was conducted to determine the oil droplets of BMSCs in the presence of caffeine, caffeine + PL, and caffeine + PL + ICI (200×). (H) Quantitative analysis of ORO staining; (I) QRT-PCR analysis was applied to analyze the expression of Pparg, Fabp4, and LPL in BMSCs after caffeine, caffeine + PL, and caffeine + PL + ICI treatment. n=3. *, P<0.05; **, P<0.01; ***, P<0.001.