Table 2. Characteristics of ddPCR outcomes of cfDNA per point in time.
| T | Moment of sample | Analysis | DNA concentration (ng/μL) | Total DNA (ng) | Allele name | Wildtype concentration (copy/μL) | Variant concentration (copy/μL) | Variant percentage | Remark |
|---|---|---|---|---|---|---|---|---|---|
| t=0 (baseline) | Baseline cfDNA | ddPCR | 2.14 | 25.70 | c.524G>A; p.R175H | 198.5 | 0 | 0 | Negative |
| t=2 (baseline + 14 w) | CRE-I | ddPCR | 2.07 | 39.12 | c.524G>A; p.R175H | 499.3 | 0 | 0 | Negative |
| t=3 (baseline + 21 w) | CRE-II | ddPCR | 2.58 | 38.70 | c.524G>A; p.R175H | 425.8 | 0 | 0 | Negative |
| t=8 (baseline + 27 m) | FU I | ddPCR | 1.76 | 3.52 | c.524G>A; p.R175H | 51.0 | 0 | 0 | Negative |
| t=9 (baseline + 30 m) | FU II | ddPCR | 2.76 | 16.56 | c.524G>A; p.R175H | 217.0 | 0 | 0 | Negative |
| WBCs-FU II | ddPCR | 3.82 | 38.20 | c.524G>A; p.R175H | 508.0 | 0 | 0 | Negative | |
| t=10 (baseline + 33 m) | FU III | ddPCR | 1.02 | 19.28 | c.524G>A; p.R175H | 234.4 | 0.74 | 0.31 | Positive |
| WBCs-FU III | ddPCR | 120 | 30.00 | c.524G>A; p.R175H | 282.0 | 0 | 0 | Negative |
TP53 p.R175H (chr17:007578406; c.524G>A). cfDNA, cell free deoxynucleic acid; CRE, clinical response evaluation; WBCs, white blood cells; FU, follow-up.