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. 2021 Aug;9(15):1242. doi: 10.21037/atm-21-3076

Figure 2.

Figure 2

The cell activity and EMT mechanism of hsa_circ_0000006 in OS cells. (A) An overexpressed or silenced elevated or decreased expression of hsa_circ_0000006 by a method based on lentivirus in HOS cells. (B,C) The capacity for proliferation was determined in HOS cells (transfected with si-circ/oe-circ or NC/vector) by using CCK-8 and colony formation assays (dyed with crystal violet, >50 cells). (D,E) Cell invasion and migration were assessed by transwell assays in HOS cells transfected with si-circ/oe-circ or NC/vector (dyed with crystal violet, 50 µm). (F) The expression levels of epithelial-to-mesenchymal transition markers were detected in HOS cells by WB. The obtained data were expressed as mean ± SD. *P<0.05, **P<0.01, ***P<0.001, #P<0.05, ##P<0.01, ###P<0.001. EMT, epithelial-to-mesenchymal transition; OS, osteosarcoma; HOS, human osteosarcoma ; NC, normal control; CCK-8, Cell Counting Kit-8; WB, western blotting.