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. 2021 Aug;9(15):1242. doi: 10.21037/atm-21-3076

Figure 3.

Figure 3

hsa_circ_0000006 was found to bind with miR-361-3p and negatively regulated its expression. (A) The DLR assay was employed to evaluate the association between hsa_circ_0000006 3'-UTR and miR-361-3p. (B) The levels of hsa_circ_0000006 and miR-361-3p enrichment in HOS cells were evaluated by the RIP assay. (C) The RE of miR-361-3p was determined by qRT-PCR in HOS cells (transfected with si-hsa_circ_0000006 or oe-hsa_circ_0000006). (D) MiR-361-3p expression in OS and nearby normal tissues was evaluated by qRT-PCR. (E) The link between the expression of hsa_circ_0000006 and miR-361-3p was evaluated by Pearson analysis. ***P<0.001; ###P<0.001. NC, normal control; DLR, dual-luciferase reporter; 3'-UTR, 3'-untranslated region; HOS, human osteosarcoma; RE, relative expression; OS, osteosarcoma.