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. 2021 Aug;9(15):1242. doi: 10.21037/atm-21-3076

Figure 5.

Figure 5

hsa_circ_0000006 enhanced the proliferative, migratory, and invasive potential of OS cells via the hsa_circ_0000006/miR-361-3p/LRIG1 axis. (A,B) Relative transcriptional and translational levels of LRIG1 in HOS cells transfected with the confirmed inhibitor, mimics, NC, si-circ, or oe-circ as determined by qRT-PCR and WB, respectively. (C,D) CCK-8 and colony formation assays were used to estimate the proliferative potential of HOS cells transfected with the indicated inhibitor, mimics, NC, si-circ, or oe-circ (dyed with crystal violet, >50 cells). (E,F) Cell invasive and migratory abilities were evaluated by transwell assays in HOS cells transfected with the indicated mimics, inhibitor, NC, si-circ, or oe-circ (dyed with crystal violet, 50 µm). (G) The expression of the epithelial marker E-cadherin was detected in HOS cells by WB. The obtained data were expressed as mean ± SD. ***P<0.001; #P<0.05; ##P<0.01; ###P<0.001; &&P<0.01; &&&P<0.001. LRIG1, immunoglobulin-like domains protein 1; NC, normal control; OS, osteosarcoma; HOS, human osteosarcoma ; WB, western blotting; CCK-8, Cell Counting Kit-8.