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. 2021 Aug;9(15):1228. doi: 10.21037/atm-21-378

Figure 6.

Figure 6

Daidzein protected AML-12 hepatocytes from Con A-induced apoptosis, and Akt phosphorylation was involved in the daidzein-mediated protective effects. AML-12 cells were seeded in 96-well plates, the cells were pretreated with daidzein (20, 40, 80, 120 µm) for 6 h and treated with Con A (10 µg/mL) for 12 h. The group without daidzein pretreatment or Con A stimulation served as the control group. (A) The effect of daidzein and LY294002 on AML-12 cell viability under normal conditions. (B) AML-12 cells were pretreated with daidzein with or without LY294002 (20 µm) and then treated with Con A for 12 h. A CCK-8 assay assessed cell viability. (C,D) The Annexin V and PI dual-labeling technique was used to analyze the effect of daidzein (80 µm) on Con A-induced AML-12 cell apoptosis. These results were obtained from at least three independent experiments. All values are expressed as the mean ± SD (n=6 per group). ***P<0.001 vs. the control group. #P<0.05, ##P<0.01 and ###P<0.001 vs. the Con A group.