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. 2021 Aug 19;13(9):e13490. doi: 10.15252/emmm.202013490

Figure 3. MSOT detection of lipid content in healthy and steatotic livers in vivo and ex vivo .

Figure 3

  1. Oil Red O staining of control (healthy) and steatotic liver. Scale bar: 100 µm.
  2. Reconstructed MSOT image (800 nm) with linear unmixing and difference value of lipid in lower abdominal section. Unmixing result: blue for Hb, red for HbO2, yellow for lipid and jet for 700–930 nm difference. The colour bar shows the colour coding of MSOT a.u. from 0 to maximum (bottom to top) (maximum value control/steatosis: Hb: 1.1/0.5; HbO2: 1.9/0.8; lipid: 21,000/24,000; 700–900 nm: 3,000/3,000). Scale bar: 4 mm.
  3. Normalized spectra of livers and kidneys from a control (healthy) and a subject with hepatic steatosis. Each spectrum is from the same animal.
  4. Reconstructed MSOT image (800 nm) with difference values of lipid in control (healthy) and steatotic liver ex vivo. The colour bar shows the colour coding of MSOT a.u. from 0 to maximum (bottom to top) (maximum value control/steatosis: 10,000/10,000). Scale bar: 4 mm.Data in panel A–D are from the same subjects.
  5. Linear unmixing readouts of lipids and difference values from control and steatotic (grade 3) livers. Control: n = 6, steatosis: n = 9. Each dot represents data from one animal (control: n = 6, steatosis: n = 9). Data represent the mean (± 95% confidence). The Mann–Whitney test and the unpaired t‐test were used to verify the statistical significance in linear and difference data, respectively. Linear liver control versus steatosis: P = 0.0004; difference liver control versus steatosis: P = 6.62E‐06.

Data information: In the figure, A.U. = arbitrary units.