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. 2021 Aug;9(16):1300. doi: 10.21037/atm-21-3746

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Immunofluorescence assay shows the proportion of Ki67-positive cells were significantly higher in the A-CM group. (A) Representative fluorescent picture of MCF-7 cells and MDA-MB-231 cells at 48 h of incubation with A-CM or U-CM (upper; magnification, 400×). (B) Corresponding quantification using the blue mean fluorescence intensity of DAPI at 12, 24, and 48 h of incubation with A-CM or U-CM (lower). (* indicates P<0.05; NC: means natural control group, which were treated with free DMEM/F12). ADSCs, adipose-derived stem cells; UMSCs, umbilical mesenchymal stem cells; A-CM, ADSCs-related medium; U-CM, UMSCs-related medium; DAPI, 4',6'-diamidino-2-phenylindole hydrochloride; DMEM, Dulbecco’s modified Eagle’s medium.