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. 2021 Aug;9(16):1348. doi: 10.21037/atm-21-4174

Figure 3.

Figure 3

circRHBG functions as a sponge for miR-515-5p. (A) Prediction of the binding site of miR-515-5p on circRHBG. (B) Luciferase activity of KGN and SVOG cells transfected with circRHBG-wt + miR-NC mimics, circRHBG-wt + miR-515-5p mimics, circRHBG-mut + miR-NC mimics, and circRHBG-mut + iR-515-5p mimics. (C) MS2-based RNA immunoprecipitation assay in KGN and SVOG cells transfected with MS2bs-circRHBG, MS2bs-circRHBGmt, or MS2bs-Rluc. (D) Expression of mir-515-5p in KGN and SVOG cells transfected with si-circRHBG and si-NC, respectively. **P<0.01.