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. 2021 Aug;9(16):1341. doi: 10.21037/atm-21-3930

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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CircMMP1 is a sponge of miR-1238 in CRC. (A) 18S, Actin, circMMP1, and MMP1 expression in nuclear and cytoplasmic fractions were analyzed in SW480 and SW620 cells by RT-qPCR. (B) Predicted binding sites of miR-1238 within the circMMP1 sequence. (C) The relative expression level of miR-1238 in CRC cells compared to normal colorectal cell NCM460. (D) Luciferase reporter assay of SW620 CRC cell co-transfected with miR-1238 mimics and the 3'-circMMP1 wild type or mutant luciferase reporter. (E) MS2-based RIP assay transfected with MS2bs-circMMP1 plasmid, MS2bs-circMMP1-mt plasmid or Rluc control plasmid. **P<0.01. CRC, colorectal cancer; RT-qPCR, quantitative reverse transcription polymerase chain reaction.