FIG. 3.

Inhibition of DMSO-induced MEL cell differentiation by expression of α-sense HERF1. MEL cells expressing the tetracycline-regulated Tet-VP16 fusion protein, stably transfected with either an empty vector (parental) or a tetracycline-regulated α-sense HERF1-containing plasmid (α sense HERF1), were treated for 3 to 5 days with DMSO in the presence (+) or absence (−) of tetracycline (tet). Following the indicated treatments, cells were isolated and assessed by Northern blot analysis (A) and morphology (B). Northern blots of total RNA were sequentially hybridized with probes specific for the genes listed on the right side.