Figure 3.

Effect of AS-IV on the activity of NO in the aortae of diabetic mice. (A) Representative photomicrographs of 4,5-diaminofluorescein diacetate-stained thoracic aorta sections from (a) control, (b) DM and (c) 3, (d) 6 and (e) 12 mg/kg DM+AS-IV groups (magnification, x200). (B) Analysis of fluorescence intensity of NO from (A). (C) Western blot analysis of eNOS and p¹¹⁷⁷ eNOS. (D) Analysis of eNOS and p¹¹⁷⁷ eNOS from (C). Data are presented as the mean ± SEM (n=4-6). ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. the control group. ^#P<0.05 and ^###P<0.001 vs. the DM group. AS-IV, Astragaloside IV; DM, diabetes mellitus; NO, nitric oxide; eNOS, endothelial nitric oxide synthase; p, phosphorylated.