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. 2021 Aug 24;22(5):1212. doi: 10.3892/etm.2021.10646

Figure 2.

Figure 2

Establishment of the high HO-1 expression model. (A) HO-1 expression exhibited time-dependent alterations. Within 4 h, HO-1 expression was increased, after which it gradually declined and reached the lowest level at 72 h. (B) Hemin induced HO-1 mRNA expression in NG + Hemin group. (C) Hemin induced HO-1 mRNA expression in AGEs + NG + Hemin group. (D) Hemin induced HO-1 mRNA expression in AGEs + HG + Hemin group. (E and F) Hemin induced HO-1 protein expression in the hemin groups, as indicated by western blotting. (G) In the AGES + NG and AGEs + HG groups, the HO-1 activity was lower than that of the NG group. (H) Hemin increased HO-1 activity, and CrMP abolished the effects of hemin in the NG group. (I) Hemin increased HO-1 activity, and CrMP abolished the effects of hemin in the AGEs + NG group. (J) Hemin increased HO-1 activity, and CrMP abolished the effects of hemin in the AGEs + HG group. All experiments were performed in triplicate, and the data are presented as the means ± SD. *P<0.05. NG, normal glucose; HG, high glucose; AGEs, advanced glycation end products; HO-1, heme oxygenase-1; CrMP, chromium mesoporphyrin.