Skip to main content
. 2021 Aug 23;22(5):1200. doi: 10.3892/etm.2021.10634

Figure 4.

Figure 4

PI3K inhibitor or CXCR4 inhibitor suppresses proliferation and migration, while accelerating apoptosis of CXCR4-mediated BM-EPCs under hypoxic conditions. BM-EPCs were divided into four groups: Hypoxia + Sham, Hypoxia + CXCR4, Hypoxia + CXCR4 + LY294002, and Hypoxia + CXCR4 + AMD3100 groups. (A) Cell Counting Kit-8 assay was used to assess the proliferation abilities of BM-EPCs. (B) Transwell assay to evaluate cell migration ability (magnification, x200). (C) Flow cytometry to analyze cell apoptosis capacity. (D) Quantification of LY294002- and AMD3100-suppressed migration of CXCR4-mediated BM-EPCs under hypoxic conditions. (E) Quantification of LY294002 and AMD3100 increased apoptosis of CXCR4-mediated BM-EPCs under hypoxic conditions. Experiments were repeated 3 times; data are expressed as mean ± SD. *P<0.05 and **P<0.01 vs. Hypoxia + Sham; #P<0.05 and ##P<0.01 vs. Hypoxia + CXCR4. BM-EPCs, bone marrow-derived endothelial progenitor cells; PI3K, phosphatidylinositol 3-kinase; CXCR4, chemokine receptor-4.