FIG. 11.

Tyrosine phosphorylation of STAT6 in response to IL-4 is enhanced in the IGF-IRWT transfectants. (A) 32D cells and transfectants were serum starved and either left untreated or stimulated with IGF-I and/or IL-4 for 10 min. Equivalent cell lysates were immunoprecipitated (IP) with anti-STAT6. Transferred proteins were immunoblotted with anti-pTyr. The position of tyrosine-phosphorylated STAT6 is indicated. This experiment was performed three times with the same results. (B) The same Immobilon membrane shown in panel A was reblotted with an anti-STAT6 antibody. The position of the STAT6 protein is indicated.