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. 2021 Sep 6;14:134. doi: 10.1186/s13041-021-00845-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Gdf11 is expressed by neuroblasts and mature neurons in the adult dentate gyrus. Representative RNAscope micrographs from a 3-month-old male C57BL/6 mouse probed for Gdf11 (white puncta) cell type specific markers for neuroblasts (Dcx, pink) or mature neurons (Map2, green) and stained with DAPI (blue). Gray boxes mark the regions chosen for the inserts below each micrograph shown. Scale bar = 20 μm. Inserts are magnified DAPI stained nuclei (grey) with each puncta outlined by either white, pink, or green. A light blue overlay outlines the perinuclear area used to determine puncta coexpression