Fig. 1.

No obvious phenotypic changes in miR-100 overexpressing mice under normal chow diet (ND). a The schematic illustration of our miR-100 breeding strategy is shown. b The applied breeding scheme resulted in a ~ 22 fold overexpression of miR-100 in liver tissue of transgenic mice as identified using stem-loop based realtime PCR. c, d CD36 and Cyp4a14 were significantly downregulated in liver tissue of transgenic miR-100 mice compared to WT control as analyzed using quantitative real-time PCR analysis. e, f The gain in weight was similar in miR-100 mice compared to WT animals under normal chow diet. g The food intake in ND was comparable in WT and miR-100 mice. h, i No significant changes in serum aspartate (AST) and alanine (ALT) transaminases. (n = 5–6 for all experiments) Data represent mean values with SEM. n.s. not significant, *P < 0.05 and ***P < 0.001 vs. the corresponding control