Fig. 2.

No metabolic changes under normal chow diet (ND) in miR-100 mice. a–d There was no difference in the weight of liver (a), brown fat tissue (b), subcutaneous fat (c) and visceral fat (d) at the end of the 16 weeks ND feeding period (age 18–19 weeks) in miR-100 overexpressing mice in comparison to the wildtype controls. e–h The expression of lipogenic genes in the liver of miR-100 mice was not significantly altered as seen by quantitative real-time PCR analysis compared to WT animals. i, j The intraperitoneal glucose tolerance test at the end of the feeding period revealed no difference in WT and miR-100 mice. k, l The intraperitoneal insulin tolerance test at the end of the feeding period showed no change in miR-100 mice compared to wildtype controls. (n = 5–6 for all experiments) Data represent mean values with SEM. n.s. not significant vs. the corresponding control