Fig. 3.

Reduced weight gain in miR-100 mice under 16 weeks high fat diet feeding. a, b High fat diet (HFD) feeding (b) (n = 6–14) increased the expression of miR-100 in wildtype and miR-100 overexpressing mice compared to normal chow diet (ND) (a) (n = 5–13). c The food intake in the HFD group was similar between WT and miR-100 mice. d The gain in weight during the 16 weeks of high fat diet feeding was reduced in miR-100 animals. e The final weight (age 18–19 weeks) was significantly different in miR-100 mice compared to WT. e–h The weight of liver (f), subcutaneous fat (h) and visceral fat (i) was significantly lower at the end of the HFD feeding period but not the amount of brown fat tissue (g). j–m Total serum cholesterol (j), triglyceride (k) and HDL (l) levels were not reduced, whereas LDL (m) showed a significant difference in miR-100 overexpressing mice. (n = 13–14) Data represent mean values with SEM. n.s. not significant, *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the corresponding control