Fig. 4.

Reduced hepatic fat accumulation in miR-100 mice under 16 weeks high fat diet feeding. a, b The level of liver cholesterol (a) was not significantly reduced, whereas triglycerides (b) were diminished in miR-100 animals. c, d Serum aspartate transaminase (AST) activity (c) was reduced in miR-100 overexpressing mice, whereas no difference was detectable in serum alanine transaminase (ALT) (d). e Representative pictures of cryo preserved liver sections (8 µm) stained for Oil Red O are shown. f The quantification of the Oil Red O positive areas was done using Image J software. Transgenic miR-100 mice displayed an improved glucose tolerance in glucose tolerance test (GTT) (g, h) as well as insulin sensitivity in insulin tolerance test (ITT) (I, j) compared to wildtype animals. (n = 13–14 for all experiments) Data represent mean values with SEM. n.s. not significant, *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the corresponding control