Fig. 5.

Hepatic lipogenic genes and proteins are decreased in miR-100 mice after 16 weeks of HFD. a–f The lipogenic gene program was robustly suppressed in miR-100 transgenic animals under HFD compared to WT as revealed by quantitative real-time PCR analysis. g Representative Western blots of key enzymes of lipogenesis (ACC1, FAS and PPARγ) and the potential miR-100 targets (CD36 and CYP4A14) are demonstrated. h–l The quantification of Western blots seen in (g) is shown. (n = 13–14 for all experiments) Data represent mean values with SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the corresponding control