Figure 1.

Schematic of the Triplex PK setup. Magnetic microspheres are coupled to NeutrAvidin, which binds to the biotin conjugated goat anti-mouse IgG. This antibody is in an orientation that allows for binding of the anti-ID antibody in the correct orientation to allow for optimal binding of the corresponding mAb. In a multiplex setup, the anti-ID antibodies are bound to the goat anti-mouse IgG Biotin in separate reactions, are washed, and then combined. PGT121, PGDM1400 and VRC07-523-LS mAbs are then added simultaneously into the solution. The microspheres are then washed, and detection antibody is added.