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. 2021 Aug 24;12:709994. doi: 10.3389/fimmu.2021.709994

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Copyright © 2021 Wesley, Chiong, Seaton, Arocena, Sawant, Hare, Hernandez, Rojas, Heptinstall, Beaumont, Crisafi, Nkolola, Barouch, Sarzotti-Kelsoe, Tomaras and Yates

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Non-specific background activity of HIV-1 seronegative human serum. 132 HIV-1 seronegative samples were tested at 1:100 in the PGDM1400/PGT121/VRC07-523-LS PK BAMA, and the distribution of binding to the PGDM1400 anti-ID (A), PGT121 anti-ID (B), and VRC07-523-LS anti-ID (C) was plotted. Normal and kernel density estimates are used to visualize the data distribution. Normal shows the fit of a normal distribution to the FI-Bkgd measurements, and a superimposed fitted density curve on the histogram. Kernel density estimates on histograms help smoothen the data distribution and help visualize the non-normal data distribution and patterns.