Table 1.

Limit of detection and quantification in HIV-1 seronegative human serum.

mAb	Serostatus	95th percentile (FI-Bkgd)	LOD ng/ml	Physiological LOD µg/ml	LLOQ ng/ml (mean +10 stdev)	Physiological LLOQ µg/ml	ULOQ ng/ml (highest concentration with accurate recovery)	Physiological ULOQ µg/ml	LLOQ ng/ml (lowest accurate point above LOD)	Physiological LLOQ µg/ml
											PGDM1400	Seronegative	1049	0.26	0.026	0.76	0.076	50	5	0.391	0.039
PGT121	Seronegative	1419	0.91	0.091	2.6	0.26	250	25	0.977	0.098
VRC07-523-LS	Seronegative	1126	0.14	0.014	0.41	0.041	37	3.7	0.152	0.098

LOD, LLOQ and ULOQ as well as the corresponding physiological concentrations observed in the PGDM1400/PGT121/VRC07-523-LS Triplex PK for HIV-1 seronegative human serum.