Table 2.
Repeatability of PGDM1400/PGT121/VRC07-523-LS standard curve.
| mAb | Conc (μg/ml) | Mean FI-Bkgd | %CV (12 curves) |
|---|---|---|---|
| PGDM1400 | 0.1 | 28526.25 | 3.92 |
| 0.05 | 28256.63 | 4.24 | |
| 0.025 | 26743.50 | 5.74 | |
| 0.0125 | 24070.25 | 8.12 | |
| 0.00625 | 16543.88 | 13.69 | |
| 0.003125 | 9521.50 | 15.43 | |
| 0.001563 | 5346.25 | 17.43 | |
| 0.000781 | 2826.75 | 17.70 | |
| 0.000391 | 1519.00 | 17.22 | |
| 0.000195 | 746.38 | 19.99 | |
| 0.000098 | 360.25 | 22.11 | |
| 0.000049 | 180.13 | 27.32 | |
| 0.000024 | 157.38 | 28.16 | |
| 0.000012 | 85.50 | 25.79 | |
| PGT121 | 0.5 | 27582.50 | 3.90 |
| 0.25 | 27293.38 | 3.94 | |
| 0.125 | 26578.63 | 3.16 | |
| 0.0625 | 24460.25 | 2.84 | |
| 0.03125 | 18272.13 | 4.46 | |
| 0.015625 | 11092.50 | 4.59 | |
| 0.007813 | 6342.63 | 6.82 | |
| 0.003906 | 3342.13 | 5.66 | |
| 0.001953 | 1720.25 | 10.49 | |
| 0.000977 | 843.13 | 7.20 | |
| 0.000488 | 393.75 | 13.52 | |
| 0.000244 | 188.13 | 23.56 | |
| 0.000122 | 144.50 | 25.80 | |
| 0.000061 | 78.38 | 29.48 | |
| VRC07-523-LS | 1 | 26137.25 | 5.03 |
| 0.333333 | 25989.13 | 3.75 | |
| 0.111111 | 24656.63 | 5.46 | |
| 0.037037 | 22778.63 | 8.45 | |
| 0.012346 | 20545.50 | 11.35 | |
| 0.004115 | 13522.25 | 11.35 | |
| 0.001372 | 6874.38 | 11.24 | |
| 0.000457 | 2803.50 | 13.58 | |
| 0.000152 | 919.63 | 16.44 | |
| 0.000051 | 372.00 | 28.22 | |
| 0.000017 | 131.38 | 65.48 | |
| 0.000006 | 50.75 | 173.37 | |
| 0.000002 | 84.25 | 66.03 | |
| 0.000001 | 48.38 | 157.62 |
The table shows the average binding (Mean FI-Bkgd) of each of the 12 titrated curves at each dilution as well as the %CV, which was less than 18% at each dilution within the linear range of the curves. Values in bold are above the FI-Bkgd LOD for the respective mAb and curve.