Table 3.
Intermediate precision of PGDM1400/PGT121/VRC07-523-LS standard curve.
| mAb | Conc (μg/ml) | Mean FI-Bkgd | %CV (3 curves) |
|---|---|---|---|
| PGDM1400 | 0.1 | 28018.88 | 1.79 |
| 0.05 | 27914.25 | 2.13 | |
| 0.025 | 26289.13 | 1.38 | |
| 0.0125 | 23187.63 | 3.55 | |
| 0.00625 | 15701.00 | 11.94 | |
| 0.003125 | 9146.88 | 12.46 | |
| 0.001563 | 5118.38 | 20.86 | |
| 0.000781 | 2746.13 | 12.31 | |
| 0.000391 | 1424.63 | 12.34 | |
| 0.000195 | 726.00 | 10.88 | |
| 0.000098 | 373.75 | 15.55 | |
| 0.000049 | 180.13 | 26.74 | |
| 0.000024 | 166.88 | 4.53 | |
| 0.000012 | 103.63 | 18.23 | |
| PGT121 | 0.5 | 27042.75 | 3.67 |
| 0.25 | 27073.38 | 2.77 | |
| 0.125 | 26298.25 | 3.13 | |
| 0.0625 | 24228.63 | 1.99 | |
| 0.03125 | 18661.75 | 4.51 | |
| 0.015625 | 11092.50 | 3.39 | |
| 0.007813 | 6351.63 | 8.40 | |
| 0.003906 | 3392.25 | 7.07 | |
| 0.001953 | 1719.13 | 8.48 | |
| 0.000977 | 877.63 | 9.35 | |
| 0.000488 | 398.75 | 15.16 | |
| 0.000244 | 201.50 | 26.83 | |
| 0.000122 | 160.00 | 13.07 | |
| 0.000061 | 84.75 | 33.69 | |
| VRC07-523-LS | 1 | 25855.00 | 5.03 |
| 0.333333 | 26093.88 | 3.75 | |
| 0.111111 | 24698.63 | 5.46 | |
| 0.037037 | 23454.38 | 8.45 | |
| 0.012346 | 21074.75 | 11.35 | |
| 0.004115 | 14348.63 | 11.35 | |
| 0.001372 | 7102.88 | 11.24 | |
| 0.000457 | 2736.75 | 13.58 | |
| 0.000152 | 940.63 | 16.44 | |
| 0.000051 | 341.63 | 28.22 | |
| 0.000017 | 173.75 | 65.48 | |
| 0.000006 | 50.75 | 173.37 | |
| 0.000002 | 120.50 | 66.03 | |
| 0.000001 | 48.38 | 157.62 |
The table shows the average binding (Mean FI-Bkgd) of each standard curve performed by the same operator over the time course of 40 days at each dilution as well as the %CV, which was less than 14% at each dilution within the linear range of the curves. Values in bold are above the LOD in FI-Bkgd for the respective mAb and curve.