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. Author manuscript; available in PMC: 2021 Sep 7.
Published in final edited form as: Mucosal Immunol. 2020 Jul 20;14(2):342–356. doi: 10.1038/s41385-020-0325-3

Figure 9: Overexpression of ADAM15 in human THP-1 cells promotes CSE-induced apoptosis and reductions in intracellular Mcl-1 protein levels.

Figure 9:

In A, ADAM15 steady state mRNA levels were measured in total cellular RNA samples from differentiated THP-1 24 h after electroporation of an ADAM15 over-expression [OE] vector (pcDNA3.1-ADAM15, a plasmid containing an ADAM15 complementary DNA) or a control vector (Vector) using quantitative real-time PCR. In B-D, differentiated THP-1 cells were electroporated with either the ADAM15 OE vector or the control vector and then incubated with 10% CSE for the time indicated. In B, loss of mitochondrial membrane potential was measured by staining the cells with JC-1 dye and quantifying the staining using MetaMorph software. In C-D, intracellular protein levels of myeloid cell leukemia-1 (MCL-1) and heat shock protein-90 (HSP90; a housekeeping control) were quantified in CSE-treated THP-1 cells using Western blotting and densitometry. The MCL-1 signals were normalized to HSP90 levels and expressed as a % of the values for controls cells incubated without CSE. Data are mean ± SD. Data were analyzed using a One-Way ANOVA followed by pair-wise testing with two-tailed Student’s t-tests. *, P < 0.03 versus the group indicated.