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. 2021 Aug 26;13(8):971–984. doi: 10.4252/wjsc.v13.i8.971

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©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non-Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/

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This schematic depicts the simplified procedure from isolation to the application of endothelial progenitor cells. Briefly, endothelial progenitor cells (EPCs) are isolated from the cord blood or adult peripheral blood and are seeded on a culture flask. After the appearance of the first colonies (around 3 wk), cells are passaged and expanded to reach the desired numbers. After several verifications, EPCs could be injected into patients with cardiovascular disorders to take advantage of their regenerative and proangiogenic properties. The graphical images were created with BioRender.com. APB: Adult peripheral blood; CB: Cord blood.