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. 2021 Sep 6;16(1):937–949. doi: 10.1515/biol-2021-0092

Figure 4.

Figure 4

miR-194-5p was a target of circ_0056618. (a) The potential binding sites between miR-194-5p and circ_0056618 are shown. (b) The expression level of miR-194-5p in HGC-27 and MKN-45 cells transfected with miR-NC or miR-194-5p was determined by qRT-PCR assay. (c and d) The luciferase activity in miR-194-5p/miR-NC and circ_0056618 WT/circ_0056618 MUT co-transfected HGC-27 and MKN-45 cells was measured by dual-luciferase reporter assay. (e and f) After RNA pull-down assay, the enrichment of circ_0056618 in HGC-27 and MKN-45 cell lysates was determined by qRT-PCR assay. (g) qRT-PCR assay was conducted for miR-194-5p expression in GES-1, HGC-27, and MKN-45 cells. (h and i) The expression levels of circ_0056618 and miR-194-5p in HGC-27 and MKN-45 cells transfected with si-NC or si-circRNA were examined through qRT-PCR assay. (j and k) After HGC-27 and MKN-45 cells were treated with control, curcumin, curcumin + vector, or curcumin + circ_0056618, the expression level of miR-194-5p was detected through qRT-PCR assay. **P < 0.01.