Clustering of samples into groups of biological replicates. Principal coordinate analysis (PCoA) of the matrix of Bray-Curtis sample dissimilarities captures differences in the diversity of the exometabolome. The tight clustering of biological replicates validates SubTap's utility for exometabolomics. (a) The exometabolome of six soil bacteria grown on LB medium varied with the duration of growth, 4 or 6 days, and strain interactions. (b) The exometabolome of six soil bacteria at 4 days on LB and NB media varied with growth medium and strain interactions. var., variance; Mono, monoculture; Cocu, coculture.