Figure 4.

Effect of thalamic FTO overexpression through AAV5-Fto microinjection into unilateral thalamus on nociceptive thresholds in naïve male mice. (A-B) Levels of Fto mRNA (A) and FTO protein (B) in the ipsilateral thalamus 5 weeks after viral microinjection. n = 5 mice/group/assay. Statistical significance was calculated with two-tailed, independent Student’s t test. t ₍₈₎ = −10.509 in A and −8.993 in B. (C-F) Paw withdrawal frequencies to 0.07 g (C) and 0.4 g (D) von Frey filament stimuli and paw withdrawal latencies to thermal (E) and cold stimuli (F) on both ipsilateral (Ipsi) and contralateral (Con) sides at the different weeks after microinjection. n = 10 mice/group. The p values on top of red diamonds indicate a difference from the baseline (0 w) of the corresponding group. Statistical significance was calculated with two-way ANOVA (treatment × time) with repeated measures followed by post hoc Tukey test. (C) Con: F_{treatment (1, 90)} = 105.313, p < 0.001; F_{time (4, 90)} = 24.397, p < 0.001; F_{treatment × time (4, 90)} = 20.603, p < 0.001. Ipsi: F_{treatment (1, 90)} = 0.0545, p = 0.816; F_{time (4, 90)} = 0.0545, p = 0.994; F_{treatment × time (4, 90)} = 0.0545, p = 0.994. (D) Con: F_{treatment (1, 90)} = 109.442, p < 0.001; F_{time (4, 90)} = 22.753, p < 0.001; F_{treatment × time (4, 90)} = 18.662, p < 0.001. Ipsi: F_{treatment (1, 90)} = 0, p = 1; F_{time (4, 90)} = 0.563, p = 0.69; F_{treatment × time (4, 90)} = 0.256, p = 0.905. (E) Con: F_{treatment (1, 90)} = 1045.843, p < 0.001; F_{time (4, 90)} = 199.553, p < 0.001; F_{treatment × time (4, 90)} = 213.138, p < 0.001. Ipsi: F_{treatment (1, 90)} = 0.381, p = 0.539; F_{time (4, 90)} = 0.759, p = 0.555; F_{treatment × time (4, 90)} = 0.736, p = 0.57. (F) F_{treatment (1, 90)} = 396.824, p < 0.001; F_{time (4, 90)} = 113.187, p < 0.001; F_{treatment × time (4, 90)} = 104.324, p < 0.001.