Table 1. Summary of DNA junction validation frequencies.
Type | Lines | In silico Assembled Junction | PCR validated Junction | Validation Frequency | Assembled Junction in Group | Validated Junction in Group | Validation Frequency in Group |
---|---|---|---|---|---|---|---|
Shattering Group | POP33_31 | 16 | 12 | 0.75 | 33 | 26 | 0.788 |
POP30_88 | 17 | 14 | 0.824 | ||||
Lesion Group | POP25_72 | 5 | 0 | 0 | 22 | 0 | 0 |
POP26_54 | 1 | 0 | 0 | ||||
POP27_88 | 14 | 0 | 0 | ||||
POP28_86 | 2 | 0 | 0 | ||||
No-lesion Group | POP27_32 | 10 | 0 | 0 | 11 | 0 | 0 |
POP27_77 | 1 | 0 | 0 | ||||
POP31_79 | 0 | 0 | 0 |
The validation frequencies represent the percentage of PCR-validated junctions out of the total number of in silico predicted junctions.