Extended Data Figure 4 ∣.

Analyses of the effects of Vitamin C deficiency on male germ cell development.

a, Unlike the consistent reduction of female germ cells, Vitamin C deficiency does not decrease the number of germ cells in E13.5 male gonad. Statistical significance assessed by two-sided Welch’s t-test. Error bars depict mean ± SD of the indicated number of biological replicates.

b, The meiotic germ cell marker SYCP3 was not identified in n=2 or n=3 male gonads with or without Vitamin C depletion, respectively. Statistical significance of E14 female gonads assessed by two-tail Mann-Whitney test. Error bars depict mean + SEM of n=6 control and n=5 -VitC biological replicates.

c, The meiotic germ cell marker STRA8 was not identified in n=2 or n=3 male gonads with or without Vitamin C depletion, respectively. Statistical significance assessed by two-tail Mann-Whitney test. Error bars depict mean + SEM. Female graph includes n=6 biological replicates.

d, Most germ cells in developing testis are proliferative (ki67+), with a few quiescent (ki67-) germ cells, and no deviations from this pattern are detected with Vitamin C deficiency. Images are representative of n=2 control and n=3 -VitC biological replicates.