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. 2021 Aug 24;12:710608. doi: 10.3389/fimmu.2021.710608

Figure 6.

Figure 6

Confirmation analysis of IFN-I genes the cortex and hippocampus of young and aged sham and TBI mice. Cortical and hippocampal expression of genes associated with IFN-I signature were assessed in young and aged sham and TBI at 24 hours post-injury. In the cortex, TBI significantly increased mRNA expression of Ifnb1 (p<0.0001, A), Irf7 (p=0.0014, B), Ifi204 (p<0.0001, C) and Isg15 (p=0.0002, D) in young and aged mice. The TBI-induced increase in Ifnb1, Ifi204, Irf7 and Isg15 was significantly elevated in the cortex of aged mice. In the hippocampus, TBI significantly increased expression of a number of IFN-I related genes in young and aged mice, Irf7 (p=0.0105, E), Ifi204 (p=0.0133, F), Isg15 (p=0.0135, G) and Mx1 (p=0.0078, H). Age also significantly increased expression of Irf7 (p=0.005, E), Ifi204 (p=0.0028, F), and Mx1 (p=0.0041, H), but there was no significant interaction between age and TBI. Age significantly increased the expression of Stat1 (p=0.0006, I) and Irf5 (p=0.0091, J), but there was no effect of TBI on either gene. No effect of age or TBI was observed on Irf1 mRNA expression (K). Data expressed as Mean ± SEM. ***p < 0.001 vs. sham (effect of TBI) and ++p < 0.01, +++p < 0.001 young TBI vs aged TBI. Two-way ANOVA using Tukey post-hoc tests, (n=7-9/group).