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. 2021 Aug 24;12:710608. doi: 10.3389/fimmu.2021.710608

Figure 7.

Figure 7

Biochemical analysis of cGAS/STING and IFN-I signaling pathways in the cortex of young and aged sham and TBI mice. Cortical protein expression of cGAS, STING and other downstream mediators of IFN-I signaling was assessed by Western immunoblotting in young and aged Sham and TBI mice at 24 hours post-injury. Representative Western blots are shown in (A). TBI increased expression of cGAS (p<0.0001, B) protein compared to sham mice. The TBI effect was significantly increased in aged mice (cGAS p=0.0007, young TBI vs aged TBI). There was no effect of TBI or age on STING (C) protein expression. The expression of pSTAT1 protein was significantly increased in the cortex of TBI mice compared to sham mice (p=0.001, D). The TBI effect was significantly increased in aged mice (p=0.0146, young TBI vs aged TBI). The expression of pSTAT3 protein was significantly increased in the cortex of TBI mice compared to sham mice (p<0.0001, E). There was no age-related effect observed. TBI significantly increased p21 expression in young and aged mice (p<0.0001, F). The TBI effect was significantly increased in aged mice (p<0.0001, young TBI vs aged TBI). Data expressed as Mean ± SEM. ***p < 0.001 vs. sham (effect of TBI) and ++p < 0.01, +++p < 0.001 young TBI vs aged TBI. Two-way ANOVA using Tukey post-hoc tests, (n=3-4/group).