Figure 4.

Selective reintroduction of PGR into AVPV kisspeptin neurons rescues the LH surge in OVX + E₂ females. (A) The occurrence of LH surges in each group, represented by the % of females demonstrating a surge after treatment with a validated E₂ positive feedback regimen known to elicit an LH surge in the afternoon but not morning time-point (n = 7-10/afternoon group and n = 4-6/morning group). The LH surge occurrence was very low in KissPRKO^mCh females and increased to WT levels in KissPRKO^Pgr females. (B) Individual dot plot of afternoon LH values showing the high proportion of animals achieving an LH surge in both WTs and KissPRKO^Pgr (Cre⁺ Pgr) females but not KissPRKO^mCh (Cre⁺ mCh) females. Dashed horizontal line designates LH surge threshold (>0.70 ng/mL). (C) Mean serum LH levels in OVX + E₂ females at the morning and afternoon time-periods demonstrating elevated surge-levels of LH in afternoon KissPRKO^Pgr but not afternoon KissPRKO^mCh females (n = 7-10/afternoon group and n = 4-6/morning group). Different letters above the bars signify significantly different (P < .05) from each other.