TABLE 1.
Conjugates for the study of nonspecific reactions
| Conjugate specificitya | Antibody | Immunoglobulin or fragment | Concn of conjugate per HRP (μg/ml) | Cross-linker(s) (source of conjugate) |
|---|---|---|---|---|
| F(ab′)2-S-AMSA-SMCC-HRP, anti-T. gondii | Mouse monoclonal | F(ab′)2 | 0.2 | S-AMSA-SMCC (Labsystems) |
| F(ab′)2-S-AMSA-SPDP-HRP, anti-T. gondii | Mouse monoclonal | F(ab′)2 | 0.2 | S-AMSA-SPDP (Labsystems) |
| Fab′-SPDP-HRP, anti-T. gondii | Mouse monoclonal | Fab′ | 0.2 | SPDP (Labsystems) |
| IgG-HRP, anti-mouse | Rabbit polyclonal | IgG | 1:1,500b | Glutardialdehyde (Dako) |
| IgG-S-AMSA-SMCC-HRP, anti-TSH (αβ) | Mouse monoclonal | IgG | 8 | S-AMSA-SMCC (Labsystems) |
| F(ab′)2-S-AMSA-SMCC-HRP, anti-HBc | Mouse monoclonal | F(ab′)2 | 1.2 | S-AMSA-SMCC (Labsystems) |
| F(ab′)2-S-AMSA-SMCC-HRP, anti-HSV-1 | Mouse monoclonal | F(ab′)2 | 2 | S-AMSA-SMCC (Labsystems) |
| Fab′-SPDP-HRP, anti-HBs | Mouse monoclonal | Fab′ | 0.5 | SPDP (Labsystems) |
| Fab′-SPDP-HRP, anti-HBs | Sheep polyclonal | Fab′ | 1 | SPDP (Labsystems) |
| IgG-HRP, anti-fluorescein | Sheep polyclonal | IgG | 1:200-1:400b | —c (DuPont, Foster City, Calif.) |
a
TSH, thyroid-stimulating hormone; HBc, hepatitis B core antigen; HSV-1, herpes simplex virus type 1; HBs, hepatitis B surface antigen.
b
The original concentration was not known; optimized dilution was used.
c
—, conjugation method not indicated.