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. 2021 May 12;67(4):241–250. doi: 10.1262/jrd.2020-108

Fig. 2.

Fig. 2.

Suppression of MS-enhanced hyperactivation by adenylate cyclase inhibitors. Percentages of motility (A, C, and E) and hyperactivation (B, D, and F) were detected after sperm were cultured for 4 h with 100 fM MS and inhibitors, including 50 and 100 μM ddAdo (A and B), 20 and 50 μM 2-CE (C and D), and 10 and 25 μM KH7 (E and F). Data represent the mean ± standard deviation (SD). (A and B) (Vehicle) medium with 0.2% (v/v) pure water as vehicle; (MS) medium with 100 fM MS and vehicle; (50 μM ddAdo) medium with 50 μM ddAdo and vehicle; (MS + 50 μM ddAdo) medium with 100 fM MS, 50 μM ddAdo, and vehicle; (100 μM ddAdo) medium with 100 μM ddAdo and vehicle; (MS + 100 μM ddAdo) medium with 100 fM MS, 100 μM ddAdo, and vehicle. (C and D) (Vehicle) medium with 0.1% (v/v) pure water and 0.1% (v/v) ethanol as vehicle; (MS) medium with 100 fM MS and vehicle; (20 μM 2-CE) medium with 20 μM 2-CE and vehicle; (MS + 20 μM 2-CE) medium with 100 fM MS, 20 μM 2-CE, and vehicle; (50 μM 2-CE) medium with 50 μM 2-CE and vehicle; (MS + 50 μM 2-CE) medium with 100 fM MS, 250 μM 2-CE, and vehicle. (E and F) (Vehicle) medium with 0.1% (v/v) pure water and 0.1% (v/v) dimethyl sulfoxide as vehicle; (MS) medium with 100 fM MS and vehicle; (10 μM KH7) medium with 10 μM KH7 and vehicle; (MS + 10 μM KH7) medium with 100 fM MS, 10 μM KH7, and vehicle; (25 μM KH7) medium with 25 μM KH7 and vehicle; (MS + 25 μM KH7) medium with 100 fM MS, 25 μM KH7, and vehicle. * indicates significant differences compared with “Vehicle” and “Inhibitors” (P < 0.05). ** indicates significant differences compared with “Vehicle,” “MS,” “Low concentration of inhibitor,” and “MS + inhibitors” (P < 0.05). # indicates significant differences compared with “Vehicle” and “MS” (P < 0.05). ## indicates significant differences compared with “Vehicle,” “MS,” “Low concentration of inhibitor,” and “MS + low concentration of inhibitor” (P < 0.05). ¢ indicates significant differences compared with “Vehicle,” “MS,” “High concentration of inhibitor,” and “MS + High concentration of inhibitor” (P < 0.05). ¢¢ indicates significant differences compared with “Vehicle,” “Inhibitors,” and “MS + Inhibitor” (P < 0.05). $ indicates significant differences compared with “Vehicle,” “Inhibitors,” and “MS + High concentration of inhibitor” (P < 0.05). $$ indicates significant differences compared with “Vehicle,” “High concentration of inhibitor,” and “MS + High concentration of inhibitor” (P < 0.05). MS, α-methylserotonin maleate; 2-CE, 2-hydroxyestradiol; ddAdo, 2’,3’-dideoxyadenosine.