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. 2021 May 12;67(4):241–250. doi: 10.1262/jrd.2020-108

Fig. 4.

Fig. 4.

Suppression of MT-enhanced hyperactivation by adenylate cyclase inhibitors. Percentages of motility (A, C, and E) and hyperactivation (B, D, and F) were determined after sperm were cultured for 4 h with 10 pM MT and inhibitors, including 50 and 100 μM ddAdo (A and B), 20 and 50 μM 2-CE (C and D), and 10 and 25 μM KH7 (E and F). Data represent the mean ± standard deviation (SD). (A and B) (Vehicle) medium with 0.1% (v/v) pure water and 0.1% (v/v) ethanol as vehicle; (MT) medium with 10 pM MT and vehicle; (50 μM ddAdo) medium with 50 μM ddAdo and vehicle; (MT + 50 μM ddAdo) medium with 10 pM MT, 50 μM ddAdo, and vehicle; (100 μM ddAdo) medium with 100 μM ddAdo and vehicle; (MT + 100 μM ddAdo) medium with 10 pM MT, 100 μM ddAdo, and vehicle. (C and D) (Vehicle) medium with 0.2% (v/v) ethanol as vehicle; (MT) medium with 10 pM MT and vehicle; (20 μM 2-CE) medium with 20 μM 2-CE and vehicle; (MT + 20 μM 2-CE) medium with 10 pM MT, 20 μM 2-CE, and vehicle; (50 μM 2-CE) medium with 50 μM 2-CE and vehicle; (MT + 50 μM 2-CE) medium with 10 pM MT, 50 μM 2-CE, and vehicle. (E and F) (Vehicle) medium with 0.1% (v/v) ethanol and 0.1% (v/v) dimethyl sulfoxide as vehicle; (MT) medium with 10 pM MT and vehicle; (10 μM KH7) medium with 10 μM KH7 and vehicle; (MT + 10 μM KH7) medium with 10 pM MT, 10 μM KH7, and vehicle; (25 μM KH7) medium with 25 μM KH7 and vehicle; (MT + 25 μM KH7) medium with 10 pM MT, 25 μM KH7, and vehicle. * indicates significant differences compared with “Vehicle,” “Inhibitors,” and “MT + Inhibitors” (P < 0.05). # indicates significant differences compared with “Vehicle” and “MT” (P < 0.05). ## indicates significant differences compared with “Vehicle,” “Inhibitors,” and “MT + High concentration of inhibitor” (P < 0.05). $ indicates significant differences compared with “Vehicle,” “MT,” “Low concentration of inhibitor,” and “MT + Inhibitors” (P < 0.05). $$ indicates significant differences compared with “Vehicle,” “MT,” and “High concentration of inhibitor” (P < 0.05). ¢ indicates significant differences compared with “Vehicle,” “MT,” “Low concentration of inhibitor,” and “MT + Low concentration of inhibitor” (P < 0.05). ¢¢ indicates significant differences compared with “Vehicle,” “MT,” “High concentration of inhibitor,” and “MT + High concentration of inhibitor” (P < 0.05). £ indicates significant differences compared with “Vehicle” and “Inhibitors” (P < 0.05). MT, 5-methoxytryptamine; 2-CE, 2-hydroxyestradiol; ddAdo, 2’,3’-dideoxyadenosine.